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Researchers have created a new gene editing tool called Retron Library Recombineering RLR that can generate up to millions of mutations simultaneously, and 'barcodes' mutant bacterial cells so that the entire pool can be screened at once. While the CRISPR-Cas9 gene editing system has become the poster child for innovation in synthetic biology, it has some major limitations. This bait-and-switch can be complicated to orchestrate, and can even be toxic to cells because Cas9 often cuts unintended, off-target sites as well. Alternative gene editing techniques called recombineering instead perform this bait-and-switch by introducing an alternate piece of DNA while a cell is replicating its genome, efficiently creating genetic mutations without breaking DNA. These methods are simple enough that they can be used in many cells at once to create complex pools of mutations for researchers to study.
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Brooklyn ImmunoTherapeutics , Inc. NYSE American: BTX "Brooklyn" , a biopharmaceutical company focused on exploring the role that cytokine-based therapy can have in treating patients with cancer, today announced the appointment of Matthew During, M. During was a pioneer in the use of viral vectors for neurological disorders, and was the first to carry out gene transfer into the brain of a human child.
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